Mei Liu
Southeast University, China
Title: In vitro evolving of a breast cancer cell-specific DNA aptamer by cell-based SELEX method and its applications
Biography
Biography: Mei Liu
Abstract
Breast cancer is a highly heterogeneous tumor with four major molecular subtypes, which has become the second leading cause of death among women
in the world. In clinic medicine, different breast cancer subtypes show distinct
response to the therapy and prognosis and the molecular classification of breast
cancer remain a challenge, which depend on highly specific molecular probes. In this study, a ssDNA aptamer with high specificity and binding affinity to SK-BR-3 cells was obtained by Cell-SELEX method. Followed in vitro studies demonstrated that the aptamer can not only distinguish SK-BR-3 breast cancer cell line from MDA-MB-231, MCF-7 breast cancer cell lines and MCF-10A human normal mammary epithelial cell line, but also can differentiate HER2-positive breast cancer tissues from Luminal A, Luminal B, triple-negative breast cancer tissues and adjacent normal breast tissues, indicating its great potential for the molecular classification of breast cancer subtypes. Moreover, the in vivo experiments of the aptamer also demonstrated its good targeting ability against tumor-bearing mice of SK-BR-3 breast cancer cells. All these results demonstrated that the aptamer could be further developed into a novel molecular probe for the precise diagnosis and a highly effective biological missile for the targeted therapy of breast cancer.